Simultaneous Optical and Electrical Recording of Single Gramicidin Channels
نویسندگان
چکیده
منابع مشابه
Simultaneous optical and electrical recording of single gramicidin channels.
We report here an approach for simultaneous fluorescence imaging and electrical recording of single ion channels in planar bilayer membranes. As a test case, fluorescently labeled (Cy3 and Cy5) gramicidin derivatives were imaged at the single-molecule level using far-field illumination and cooled CCD camera detection. Gramicidin monomers were observed to diffuse in the plane of the membrane wit...
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Visual awareness, using a number of perceptual paradigms such as binocular rivalry and visual detection, has been studied for decades. Most researches focused on how activation of brain components, in particular, the cortex changes in line with the organism’s level of awareness. However, little emphasis is put on the autonomic nervous system (ANS) in response to transitions to and from awarenes...
متن کاملGramicidin channels are internally gated.
Gramicidin channels are archetypal molecular subjects for solid-state NMR studies and investigations of single-channel or cation conductance. Until now, the transitions between on and off conductance states have been thought, based on multichannel studies, to represent monomer <--> dimer reactions. Here we use a single-molecule deposition method (vesicle fusion to a planar bilayer) to show that...
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در این پایان نامه به بررسی روش های آشکارسازی در سیستم های mc-cdma می پردازیم. با توجه به ماهیت آشکارسازی در این سیستم ها، تکنیک های آشکارسازی را می توان به دو دسته ی اصلی تقسیم نمود: آشکارسازی سیگنال ارسالی یک کاربر مطلوب بدون در نظر گرفتن اطلاعاتی در مورد سایر کاربران تداخل کننده که از آن ها به عنوان آشکارساز های تک کاربره یاد می شود و همچنین آشکارسازی سیگنال ارسالی همه ی کاربران فعال موجود در...
Tandem Gramicidin Channels Cross-linked by Streptavidin
The interaction of biotin-binding proteins with biotinylated gramicidin (gA5XB) was studied by monitoring single-channel activity and sensitized photoinactivation kinetics. It was discovered that the addition of streptavidin or avidin to the bathing solutions of a bilayer lipid membrane (BLM) with incorporated gA5XB induced the opening of a channel characterized by approximately doubled single-...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2003
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(03)74881-4